|
KMID : 0869020050080020101
|
|
Journal of Korean Orthopaedic Research Society
2005 Volume.8 No. 2 p.101 ~ p.110
|
|
|
OPG Inhibits PMMA Induced Osteoclastogenesis and NF-kappaB Activation in Osteoclast Precursor Cells
|
|
Kim Yong-Sik
Kwon Soon-Yong
Choi Nam-Yong
Han Suk-Ku
Ok Ji-Hoon
|
|
|
Abstract
|
|
|
Purpose: We investigate the effect of osteoprotegerin (OPG) on activation of osteoclastogenesis and NF-kappaB activation by PMMA (Polymethyl methacrylate) particles in osteoclast precursor cells.
Materisla and Methods:Osteoclast precursor cells (CSF-1 dependent) were obtained from whole bone marrow of C57BL mouse. Four experiments included 1) different dose of RANKL (Receptor Activator of NF-kappaB ligand) treatment (0, 1, 10, 40 ng/ml) 2) PMMA treatment +/- RANKL 3) PMMA treatment with different dose of RANKL 4) PMMA treatment +/- OPG. After treatments, cultured cells were stained with TRAP (Tartrate resistant alkaline phosphatase). The activity of NF-kappaB DNA nuclear translocation was detected by EMSA (electrophoretic mobility shift assay).
Results:The experiments with RANKL on osteoclast precursors differentiation demonstrated a dose-dependent stimulation of osteoclastogenesis (p<0.05). Control cultures without RANKL had no osteoclasts, while maintenance in 1 ng/ml of RANKL results in low level osteoclast formation. PMMA particles activated osteoclastogenesis in RANKL-primed osteoclast precursor cells. And the effect of particles on osteoclastogenesis were dependent on RANKL concentration (p<0.03). OPG treatment significantly decreased osteoclast formation and NF-kappaB DNA binding activity by PMMA particles in osteoclast precursor cells.
Conclusion:OPG inhibits activation of osteoclast formation and NF-kappaB DNA binding activity by PMMA particles through RANK-RANKL pathway.
|
|
|
KEYWORD
|
|
|
OPG, RANKL, PMMA particles, Osteoclastogenesis, NF-kB, TRAP stain, Electrophoretic mobil-ity shift assay
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|